Single Cell Transcriptomics

Silencing a single gene in Kelly Neuroblastoma Cells with CRISPR-Cas9. Cells are not synchronized so the Cell Cycle can be a confounding effect:

1 thought on “Single Cell Transcriptomics

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    Gene Ontology (GO) term enrichment is a technique used to identify which GO terms are over-represented or under-represented in a given set of genes. In single-cell analysis input list of genes of interest can be selected based on differentially expressed genes or groups of genes generated from biclustering. The number of genes annotated to a GO term in the input list is normalised against the number of genes annotated to a GO term in the background set of all genes in genome to determine statistical significance.

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